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The influence of MCMV infection on the activation marker expression on DCs and macrophages from the spleen (A) The proportion of CD80 + , CD86 + , iNOS + , and MHC class II + cells among CD169 + macrophages (Mac), <t>CD209b</t> + Mac, DCs, and red pulp macrophages of the spleens from MCMV-uninfected (blue) and MCMV-infected (red) mice. (B) The proportion of DCs and macrophage subsets (CD169 + , CD209b + , and red pulp macrophages) within parent populations. Gating strategies are shown in . (C) The MFI of CD45 in live Lin − cells from MCMV-uninfected and MCMV-infected mice; Lin, lineage markers, including CD3, CD19, CD49d, and Ly6G. Mann-Whitney test was used to compare the two groups. The boxplot indicates the median value with the interquartile range (IQR). The upper whisker extends to the largest value no further than 1.5× IQR from the hinge, and the lower whisker extends from the hinge to the smallest value at most 1.5× IQR from the hinge. n = 6 mice per group. MFI, mean fluorescence intensity. See also and .
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The influence of MCMV infection on the activation marker expression on DCs and macrophages from the spleen (A) The proportion of CD80 + , CD86 + , iNOS + , and MHC class II + cells among CD169 + macrophages (Mac), <t>CD209b</t> + Mac, DCs, and red pulp macrophages of the spleens from MCMV-uninfected (blue) and MCMV-infected (red) mice. (B) The proportion of DCs and macrophage subsets (CD169 + , CD209b + , and red pulp macrophages) within parent populations. Gating strategies are shown in . (C) The MFI of CD45 in live Lin − cells from MCMV-uninfected and MCMV-infected mice; Lin, lineage markers, including CD3, CD19, CD49d, and Ly6G. Mann-Whitney test was used to compare the two groups. The boxplot indicates the median value with the interquartile range (IQR). The upper whisker extends to the largest value no further than 1.5× IQR from the hinge, and the lower whisker extends from the hinge to the smallest value at most 1.5× IQR from the hinge. n = 6 mice per group. MFI, mean fluorescence intensity. See also and .
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The influence of MCMV infection on the activation marker expression on DCs and macrophages from the spleen (A) The proportion of CD80 + , CD86 + , iNOS + , and MHC class II + cells among CD169 + macrophages (Mac), CD209b + Mac, DCs, and red pulp macrophages of the spleens from MCMV-uninfected (blue) and MCMV-infected (red) mice. (B) The proportion of DCs and macrophage subsets (CD169 + , CD209b + , and red pulp macrophages) within parent populations. Gating strategies are shown in . (C) The MFI of CD45 in live Lin − cells from MCMV-uninfected and MCMV-infected mice; Lin, lineage markers, including CD3, CD19, CD49d, and Ly6G. Mann-Whitney test was used to compare the two groups. The boxplot indicates the median value with the interquartile range (IQR). The upper whisker extends to the largest value no further than 1.5× IQR from the hinge, and the lower whisker extends from the hinge to the smallest value at most 1.5× IQR from the hinge. n = 6 mice per group. MFI, mean fluorescence intensity. See also and .

Journal: iScience

Article Title: The influence of murine cytomegalovirus infection on susceptibility to mycobacterial infection

doi: 10.1016/j.isci.2026.115518

Figure Lengend Snippet: The influence of MCMV infection on the activation marker expression on DCs and macrophages from the spleen (A) The proportion of CD80 + , CD86 + , iNOS + , and MHC class II + cells among CD169 + macrophages (Mac), CD209b + Mac, DCs, and red pulp macrophages of the spleens from MCMV-uninfected (blue) and MCMV-infected (red) mice. (B) The proportion of DCs and macrophage subsets (CD169 + , CD209b + , and red pulp macrophages) within parent populations. Gating strategies are shown in . (C) The MFI of CD45 in live Lin − cells from MCMV-uninfected and MCMV-infected mice; Lin, lineage markers, including CD3, CD19, CD49d, and Ly6G. Mann-Whitney test was used to compare the two groups. The boxplot indicates the median value with the interquartile range (IQR). The upper whisker extends to the largest value no further than 1.5× IQR from the hinge, and the lower whisker extends from the hinge to the smallest value at most 1.5× IQR from the hinge. n = 6 mice per group. MFI, mean fluorescence intensity. See also and .

Article Snippet: CD209b (SIGN-R1) Antibody, anti-mouse, REAfinityTM , Miltenyi Biotec , Cat#130-117-786; RRID: AB_2727966.

Techniques: Infection, Activation Assay, Marker, Expressing, MANN-WHITNEY, Whisker Assay, Fluorescence

Journal: iScience

Article Title: The influence of murine cytomegalovirus infection on susceptibility to mycobacterial infection

doi: 10.1016/j.isci.2026.115518

Figure Lengend Snippet:

Article Snippet: CD209b (SIGN-R1) Antibody, anti-mouse, REAfinityTM , Miltenyi Biotec , Cat#130-117-786; RRID: AB_2727966.

Techniques: Virus, Variant Assay, Recombinant, Flow Cytometry, Purification, Staining, Saline, Enzyme-linked Immunospot, Modification, Software